Invitron C-Peptide Assay

The Invitron C-peptide Assay is an immunometric assay for the quantification of C-peptide in human plasma samples. C-peptide is a product of the metabolic processing of proinsulin to insulin by the pancreatic β-cell. It is produced and released in equimolar amounts in relation to insulin, though its significantly greater half-life leads to relatively high peripheral plasma concentrations. Traditionally, C-peptide assays have been used in differential diagnosis in hypoglycaemic patients, where a lack of suppression of C-peptide secretion is suggestive of insulinoma. Conversely, suppressed levels of C-peptide in patients with elevated insulin may indicate factitious hypoglycaemia induced by exogenous insulin administration. Though not biologically active itself, C-peptide serves as a valuable marker of pancreatic function. C-peptide measurement can provide valuable information in both non-insulin dependent as well as insulin dependent diabetics and is thus useful in the monitoring of type 2 diabetes.

This assay has been designed to operate over the wide range of circulating C-peptide concentrations that occur in healthy subjects as well as in patients with type 1 or type 2 diabetes.

Key Features
· Results in under 3 hours
· Easy to use
· Non-isotopic - high-sensitivity endpoint with no radioactive labels
· High specificity - negligible cross reactivity with Insulin (0%) and Proinsulin (2%).
· Sensitive to 5 pmol/l.
· Wide working range, 0-6000 pmol/l, reducing the need for regular sample dilution.

 Assay Protocol