CgA ELISA Kit
Assay Characteristics
| Format | 96 well plate |
| Specimen | serum or plasma |
| Sample volume | 25μl |
| Working range | 0 – 800 ng/ml |
| Sensitivity | ng/ml |
| Incubations | 1 x 2 hours, 1 x 1 hour, 1 x 30 min |
| Product code | IV5-101E |
Key Features
The Invitron CgA ELISA is an immunometric assay for the
quantitative measurement of Chromogranin A in human samples.
Key features include:
High specificity assay - employing 2 monoclonal antibodies
Simple assay protocol - predilution of samples is not necessary within the working range of this assay
Protocol
add 100 μl sample buffer
and 25 μl sample to each well
incubate for 2 hours
wash 3 times and add HRP conjugate
incubate for 1 hour
wash 3 times and add substrate
incubate for 30 min
add stop solution and read at 450nm