Insulin ELISA Kit


Assay Characteristics

Format 96 well plate
Specimen serum or plasma
Sample volume 25μl
Working range 0 – 250mU/l
Sensitivity mU/l
Specificity Insulin 100%
Proinsulin 1.2%
C-Peptide 0%
Incubations 1 x 2 hours, 1 x 30 min
Product code IV2-101E

Key Features

The Invitron Insulin ELISA is an immunometric assay for the quantitative measurement of insulin in human samples.
Key features of the assay include:
- High specificity
- Therapeutic insulin analogues can be measured using this assay
- Calibrated against WHO International Standard (IRP 66/304)

Also available:

Protocol

add 100 μl enzyme conjugate and 25 μl sample to each well
incubate for 2 hours at 37°C
wash 3 times and add substrate
incubate for 15 min at room temp
add stop solution and read at 450nm