Insulin ELISA Kit
Assay Characteristics
| Format | 96 well plate |
| Specimen | serum or plasma |
| Sample volume | 25μl |
| Working range | 0 – 250mU/l |
| Sensitivity | mU/l |
| Specificity | Insulin 100% Proinsulin 1.2% C-Peptide 0% |
| Incubations | 1 x 2 hours, 1 x 30 min |
| Product code | IV2-101E |
Key Features
The Invitron Insulin ELISA is an immunometric assay for the
quantitative measurement of insulin in human samples.
Key features include:
High specificity
Therapeutic insulin analogues can be measured using this assay
Calibrated against WHO International Standard (IRP 66/304)
- Instructions for use.pdf
- Sample publications
- Insulin and proinsulin measurements in T2 diabetes.pdf
- How to order
Protocol
add 100 μl enzyme conjugate
and 25 μl sample to each well
incubate for 2 hours at room temp
wash 3 times and add substrate
incubate for 30 min at room temp
add stop solution and read at 450nm