Invitron Intact Proinsulin Assay
The Invitron Intact Proinsulin
Assay is an
immunometric
assay for the quantification of intact proinsulin in human plasma.
Proinsulin is the initial product of pancreatic β-cell activity. Under
normal circumstances, virtually all proinsulin is cleaved at residues
32-33 and 65-66 to produce insulin and C-peptide which are subsequently
released from secretory granules. Relatively little unmodified
proinsulin finds its way into the circulation in normal subjects. An
increased fasting plasma proinsulin concentration in a hypoglycaemic
patient may be indicative of insulinoma.
In type 2 diabetes, progressive β-cell dysfunction is evidenced by an
increased ratio of proinsulin/insulin in peripheral blood. Recently, it
has been proposed that fasting intact proinsulin is a highly
specific marker for insulin resistance. Such information may be useful
in choosing the most appropriate therapy for the diabetic patient.
Historically, it was recognised that many insulin assays suffered
significantly from the problem of cross reactivity with intact and
split proinsulins. The Invitron product employs monoclonal antibodies
of
well defined reactivity to minimise problems due to cross reactivity.
Key Features
· Results in
under 4 hours
· Easy to use
· Non-isotopic - High-sensitivity endpoint
with no radioactive
labels
· High
specificity - no cross
reactivity with insulin or c-peptide
· High sensitivity of detection (0.2pmol/l)
Assay Protocol
