Intact Proinsulin Luminescence Assay Kit
Assay Characteristics
| Format | 96 well plate |
| Specimen | plasma (recommended) or serum |
| Sample volume | 50μl |
| Working range | 0 – 100 pmol/l |
| Sensitivity | 0.02 pmol/l |
| Specificity |
Proinsulin 100% Insulin 0% C-Peptide 0% 32-33 split proinsulin: 5.6% Des 31-32 split proins: 1.4% 65-66 split proinsulin: 37%* Des 64-65 split proins: 63%* *not present at detectable levels in human samples |
| Incubations | 1 x 2 hours, 1 x 1 hour |
| Product code | IV2-002 |
Key Features
The Invitron Intact Proinsulin Assay is an immunometric assay for the quantitative measurement of intact proinsulin in human samples.
Key features include:
Easy to use - very simple assay protocol
High sensitivity detection system
High specificity
Highly specific marker for insulin resistance in type 2 diabetes
Calibrated against WHO International Standard (IRP 84/611)
- Cross reactivity table.pdf
- Instructions for use.pdf
- Sample Publications
- Insulin and proinsulin measurements in T2 diabetes.pdf
- How to order
Protocol
add 50 μl sample buffer and 50 μl sample to each well
incubate for 2 hours at 37°C
wash 3 times and add 100 μl labelled antibody solution
incubate for 1 hour at 37°C
wash 3 times
read in luminometer