Invitron Total Proinsulin Assay
The Invitron Total Proinsulin
Assay is an
immunometric
assay for the quantification in peripheral blood of the major precursor
molecules produced during insulin biosynthesis by the pancreatic
β-cell. Under normal circumstances, virtually all proinsulin is cleaved
at residues 32-33 and 65-66 to produce insulin and C-peptide which are
subsequently released from secretory granules. Relatively little
unmodified proinsulin finds its way into the circulation in normal
subjects. In patients with type 2 diabetes, relatively greater
proportions of intact proinsulin, 32-33 split proinsulin and des 31-32
split proinsulin find their way into peripheral blood. It is not
thought that 65-66 split proinsulin or des 64-65 split proinsulin occur
to any significant extent in peripheral blood. These changes in the
profile of β-cell products reflect the progressive secretory
dysfunction characteristic of type 2 diabetes, with severely elevated
levels indicating an advanced stage of β-cell dysfunction.
Key Features
· Results in
under 4 hours
· Easy to use
· Non-isotopic - high-sensitivity endpoint
with no radioactive
labels
· High
specificity - no cross
reactivity c-peptide
· High sensitivity of detection
Assay Protocol
