Total Proinsulin Luminescence Assay Kit
Assay Characteristics
| Format | 96 well plate |
| Specimen | serum or plasma |
| Sample volume | 25μl |
| Working range | 0 – 250mU/l |
| Sensitivity | 0.25 mU/l |
| Specificity | Proinsulin 100% Insulin 2.2% C-Peptide 0% 32-33 split proinsulin: 97% Des 31-32 split proins: 100% 65-66 split proinsulin: 12%* Des 64-65 split proins: 1%* |
| Incubations | 1 x 2 hours, 1 x 1 hours |
| Product code | IV2-003 |
Key Features
The Invitron Total Proinsulin Assay is an immunometric assay for the quantitative measurement of total proinsulin in human samples.
Key features include:
Easy to use - very simple assay protocol
High sensitivity detection system
High specificity
Calibrated against WHO International Standard (IRP 84/611)
Protocol
add 50 μl sample buffer and 50 μl sample to each well
incubate for 2 hours at 37°C
wash 3 times and add 100 μl labelled antibody solution
incubate for 1 hour at 37°C
wash 3 times
read in luminometer